DAVID Documentation: Ortholog

DAVID Ortholog enables cross-species functional analysis by converting a source gene list into an orthologous list in a user-selected target species. Ortholog mapping data from Orthologous MAtrix (OMA) and Ensembl Compara are integrated into the DAVID Knowledgebase through DAVID Gene identifiers,

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

1. Current Gene List & Population

   Displays the active gene list and selected species or background population.
2. Help and Switch to Classic Version

   Link to this help page and provides access to the legacy Gene List Report interface.
3. Ortholog Taxonomy

   Click the dropdown box to select a target ortholog taxonomy (species). After clicking on the box, a search box is also available to type and search for taxonomies. Click on the taxonomy to select, then the ortholog sources will populate.
4. Species

   Click the Species link to go to NCBI taxonomy entry.
5. OMA

   Shows the percentage of genes from the list with an ortholog available for the selected taxonomy from Orthologous Matrix (OMA) Click the percentage bar to retrieve orthologs from that source.
6. Ensembl

   Shows the percentage of genes from the list with an ortholog available for the selected taxonomy from Ensembl Compara. Click the percentage bar to retrieve orthologs from that source.
7. Load to DAVID

   Load selected orthologs into a DAVID list for downstream analysis.
8. Select All

   Select All Multi-Orthologs will select all available orthologs. Click again to Deselect all. Click again to select only the first ortholog if there are multiple available (default).
9. Show

   Show/Hide all multi-orthologs
10. Sortable Columns

    Click any column header to sort by User ID, Expression Value, DAVID Gene Name, Related Genes (RG), or Species.
11. Gene name

    Click the gene name link to explore the full gene report. For more information, see the Gene Report documentation page.
12. Ortholog count

    Number of ortholog genes available for the user gene in the selected taxonomy.
13. Expand

    Show/Hide all multi-orthologs
14. Select

    Selected orthologs are loaded as a new DAVID list when clicking the Load ortholog list into DAVID for analysis button.
15. Ortholog Gene name

    Click the gene name link to explore the full gene report. For more information, see the Gene Report documentation page.
16. Identity

    Sequence identity between user gene and ortholog gene. Higher meaning more identical.
17. Confidence

    Confidence score. Only available for Ensembl results.